葡萄A病毒外壳蛋白基因克隆及分子变异分析

为获得葡萄A病毒(Grapevine virus A,GVA)外壳蛋白(CP)基因,并为开展GVA-CP介导的抗病毒转基因研究提供基础,本研究从葡萄休眠枝条中扩增获得12个GVA分离物的CP基因序列,全长597nt,编码198个氨基酸。所得分离物间核苷酸和氨基酸序列同源性分别为83.9%~99.8%和92.4%~99.5%,与GenBank中12个GVA CP序列比较,核苷酸和氨基酸序列同源性分别为71.0%~93.1%和81.8%~98.0%。系统进化分析表明,本研究12个分离物CP序列与已报道的GVA Ⅰ组亲缘关系最近。种群变异分析表明,GVA种群具有多种变异体类型,并且多个样品存在不同变异体类型的复合侵染。但绝大多数变异体克隆聚集在GVA Ⅰ组分支,有60%的克隆序列间同源性较高,并且聚集在同一个次级分支Ⅰ Aa,表明其为该GVA种群的优势序列。克隆的GVA CP基因以及优势序列群体的分析为葡萄抗病毒转基因研究奠定了基础。     

用基于PCR的分子标记分析甘蓝型油菜抗感菌核病品系间的遗传距离

用离体叶接种法鉴定了甘蓝型油菜选系对菌核病的抗感性，结果表明绝大多数抗性品系与感病品种间的差异达到显著或极显著水平。以两种基于ＰＣＲ的标记估计所选１０ 份材料间的遗传距离。１９ 个随机引物扩增出了２６ 条重复性很好的ＲＡＰＤ 标记，１ 对依据植物Ｒ基因的ＮＢＳ区段设计的简并引物扩增出了３ 条多态性的带。依据２９ 个标记进行聚类分析，结果表明抗、感性材料在遗传距离为０．１２０６ 处被明显地区分开。５份抗性材料中，宁ＲＳ—１ 与中油８２１、中Ｒ８８８ 等其它抗性品系的遗传距离较远     

Genetic diversity of apricot revealed by a set of SSR markers from linkage group G1

Eight polymorphic simple sequence repeat (SSR) markers located in the G1 linkage group of apricot (Prunus armeniaca L.) were previously developed and evaluated in a small set of cultivars. Those primers were used for studying variability in 77 apricot cultivars belonging to five different geographical groups, such as Chinese, Asian (Irano-Caucasian and Central Asian), North American, Mediterranean and Western European as well as Middle European cultivars. Six of the markers were polymorphic and revealed a total of 71 alleles ranging from 5 (aprigms11) to 20 (aprigms1) alleles per locus with a mean value of 11.83 alleles per locus. In conclusion, the SSR loci located in the G1 linkage group show a level of polymorphism which is similar to loci dispersed throughout the entire genome. The total number of alleles and the number of unique alleles were the highest in Chinese apricots and the lowest in Middle European cultivars. Heterozygosity also showed a decrease from Asia and China to Middle Europe. No association could have been observed between any SSR markers tested and plum pox virus (PPV) resistant phenotype of cultivars. PPV resistant cultivars did not form a separate clade on the dendrogram obtained by UPGMA cluster analysis. Middle European and Chinese cultivars formed separate clusters while other genotypes formed smaller multiple sub-groups or scattered among different clusters. Our results support previous hypotheses on the origin of PPV resistance in North American apricots. The allele data was also presented in a form that allowed the easy observation of allele frequencies in each geographical group at each locus. Using this data field, differences and similarities between cultivar groups can be easily assessed. The analysis demonstrated the links between the North American and Mediterranean apricot germplasm and confirmed that the Chinese and Eastern European cultivars are distantly related.     

高等植物小孢子胚胎发生的细胞和分子生化机制研究进展

小孢子胚胎发生的细胞学研究已取得了显著进展,并分离鉴定出一些小孢子胚胎发生表达的胚胎专一基因,但小孢子胚胎发生的机理尚未完全明确。综述了高等植物小孢子胚胎发生的细胞学以及分子和生物化学方面的最新研究进展,旨为进一步揭示小孢子的胚胎发生机理提供十分有用的信息。     

Intron Polymorphisms： Species-specific and Interspecies-comparable Markers

As a component of genes, introns are much more variable than exons. Therefore, introns are an important source of genetic polymorphisms and can be used as genetic markers. By desingning PCR primers in the two exons flanking an intron, the intron can be specifically amplified. There are mainly two types of intron polymorphisms： intron length polymorphism （ILP, due to InDel or SSR） and SNP. ILP can be conveniently detected by electrophoresis, while SNP can be detected by sequencing, ECOTILLING or other methods.     

核果类果树遗传连锁图谱的研究进展

遗传连锁图谱构建是基因组研究中的重要环节,是基因定位与克隆乃至基因组结构与功能研究的基础。近20a来,分子生物学特别是分子标记技术的飞速发展为高饱和核果类遗传连锁图谱的构建和利用奠定了基础。目前国内外已经公布十几张核果类的遗传连锁图谱,但这些图谱均有不同的缺陷。因此,我们就核果类遗传连锁图谱的构建进展、性状的QTL定位及比较图谱应用进行了综述,并探讨了核果类遗传连锁图谱研究的前景和存在的问题。     

封育年限对典型草原大针茅无性系构件组成与生长的影响

研究不同封育年限对黄土高原大针茅(Stipa grandis)无性系构件结构组成和生长的影响,可为阐明无性系构件组成和资源分配提供一定基础。在黄土高原典型草原选取不同封育年限区(10和20年)和放牧地(对照)作为试验样地,采用整个无性系完整挖掘的方法进行大针茅无性系构件特征的研究。结果表明：1)与放牧相比,封育10年显著增加了大针茅无性系丛径、生殖枝高度和花序高度(P < 0.05),而封育20年则显著提高了大针茅无性系丛径、总枝数、生殖枝数、营养枝数、分蘖芽数、生殖枝高度、花序高度、地上总生物量、营养枝生物量和花序生物量(P < 0.05)。封育降低了大针茅生殖枝数、生殖枝高度、花序高度、地上总生物量、生殖枝生物量和花序生物量的变异系数。2)随封育年限增加,营养枝生物量比例明显提高,生殖枝生物量比例下降,但花序生物量比例基本不变。3)封育措施显著提高了营养株单株生物量(P < 0.05),但生殖株单株生物量和全体分枝单株生物量与放牧处理差异不显著(P > 0.05)。4)大针茅丛径与地上总生物量、生殖枝数、生殖枝生物量、花序生物量呈极显著正相关关系(P < 0.001),与营养枝生物量呈极显著正相关关系(P < 0.01),与总枝数呈显著正相关关系(P < 0.05)。而地上总生物量与生殖枝数、营养枝数、总枝数、营养枝生物量呈极显著正相关关系(P < 0.001),与分蘖芽数、生殖枝生物量和花序生物量呈极显著正相关关系(P < 0.01)。分蘖芽数与营养枝数、总枝数、营养枝生物量呈极显著正相关关系(P < 0.001),与地上总生物量呈极显著正相关关系(P < 0.01)。综上所述,短期封育提高了大针茅的生殖分配,使大针茅迅速成为群落优势种,而长期封育使大针茅更依靠无性繁殖进行种群更新。     

中国驴品种资源现状及其分子遗传育种研究进展

驴是中国一个古老畜种,对不良环境适应性强,耐粗饲、抗病力强,一直是役、驮、乘、肉、药多用资源之一。但随着农业机械化、运输业快速发展及人民生活水平提高,近些年来驴品种资源整体面临着种质资源退化、种群数量和质量显著下降等问题。本文针对我国驴品种资源概况、保护利用现状、存在问题及其分子遗传育种研究进展进行综述,以期为驴品种资源的保护及开发利用提供参考。     

贵州苏铁研究进展

本文对贵州苏铁研究在形态特征、生物地理学、生殖生物学特性、细胞学和分子生物学等几个方面开展的科研工作进行综述。     

Comparison of chitosans with different molecular weights as possible wood preservatives

Fungi cause serious problems in wood utilization, and environmentally benign wood protection is required as an alternative to traditional chemicals. Chitosan has shown promising antimicrobial properties against several microorganisms. In this study, we present the characterization of and antifungal properties of a commercial chitosan formulation developed for impregnation of wood. A broad range of chemical and mycological methods were used to evaluate the uptake, fixation, and antifungal properties of chitosan for wood preservation. The results show that the higher the uptake of chitosan the lower the relative recovery of chitosan in wood after leaching, and the higher the molecular weight of chitosan the higher the recovery. Chitosan with high molecular weight proved to be more efficient against decay fungi than chitosan with low molecular weight. The fungi tested on chitosan-amended nutrient agar medium were totally inhibited at 1% (w/v) concentration. In decay studies using small wood blocks, 4.8% (w/v) chitosan concentration gave the best protection against brown rot fungi.